Yeast DNA Isolation Mini Kit
Yeast DNA Isolation Mini Kit
Yeast cells have a hard cell wall that hinders researchers from quickly and effectively separating and extracting genomic DNA from yeast cells. This kit provides Lyticase that can efficiently hydrolyze yeast cell walls and a centrifuge adsorption column that can specifically bind to DNA for extracting genomic DNA from yeast cells. Using Lysis Solution to lyse cells, adding ethanol, and directly adding the solution to a centrifuge column that can efficiently and specifically adsorb DNA, can ma💯ximize the removal of impurities such as protein.
Product Characters
● The DNA extraction process does not require phenol chloroform extraction and does not contain ethanol precipitatiꦇon steps
● It uses lysis separation technology to remove proteins. The entire p♔urification process can generally be completed within 1 hour
● All the silicon matrix membranes in the centrifugal adsorption column are made of specially designed adsorption membranes, with minimal differenc🎀es in adsorption capacity between columns and good repeatability
● Multiple column rinsing steps can ensure the high purity of yeast genome extraction. The typical OD260/280nm ratio of🀅 the product is 1.7-1.9, with a length of 50-10♐0kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions
Kit Components
Components | GK1091 | GK1092 |
gDNA Recovery Column | 50 pieces | 100 pieces |
2.0-ml Collection Tube | 50 pieces | 100 pieces |
Boiled RNase A | 250 μl | 500 μl |
Proteinase K | 1.2 ml | 1.2 ml*2 |
Lyticase | 250 μl | 500 μl |
LysisY buffer | 35 ml | 70 ml |
TBM | 12.5 ml | 30 ml |
12.5 ml | 30 ml | |
Wash Solution | 12 ml | 24 ml |
Elution Buffer | 10 ml | 20 ml |
Buffer CBS | 15 ml | 25 ml |
Protocol | 1copy | 1copy |
