Plant Genomic DNA Isolation Mini Kit
Plant Genomic DNA Isolation Mini Kit
The improved CTAB plant DNA extraction solution (with components added to remove polysaccharides and polyphenols) rapidly cleaves cells and inactivates intracellular nucleases. After chloroform extraction, polysaccharides, polyphenols, and prot♉eins are removed by centrifugation. Add isopropanol to the supernatant and centrifuge to precipitate genomic DNA, further removing various other impurities. Then, the genomic DNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as polysaccharides, polyphenols, cell metabolites, proteins, etc. are further removed. Finally, the genomic DNA is eluted from the silica matrix membrane using a low salt elution buffer.
Product Characters
● Speci💯ally designed adsorꩵption membrane with good experimental repeatability
● Fast and simple steps. Singl🐎e sample operation can generally be completed within 1🐈 hour
● Add various ingredients to remove polysaccharides and polyphenols. Multiple column rinsing st♏eps can ensure the production of high-purity DNA. The typical ratio of OD260/OD280 is 0.7-1.9, and the DNA length can reach 30kb-50kb, which can be directly used for PCR, Southern blot, and various enzyme digestion reactions
Kit Components
Components | 存储的温度 | GK1051 |
Separating Column A | Room Temperature | 50 pieces |
gDNA recovery Column AC | Room Temperature | 50 pieces |
Collection Tube（2ml） | Room Temperature | 50 pieces |
Equilibrium Solution | Room Temperature | 25 ml |
Buffer P1 | -20℃（Long term）；Room Temperature（1-3 months） | 30 ml |
Buffer P2 | -20℃（Long term）；4℃（1 month） | 7 ml |
Buffer P3 | -20℃（Long term）；4℃（1 month） | 50 ml |
RNase A | -20℃ | 200 ul |
Rinsing Solution WB | Room Temperature | 15 ml |
Elution Solution EB | Room Temperature | 15 ml |
Protocol | 1copy | 1copy |
