Comparison Table Of Synthetic Spe⛄cifications And Final Yield(15-60nt)
Purification Method | Synscale | Final Yield |
OPC | 50nmol | 2OD |
100nmol | 5OD | |
200nmol | 10OD | |
PAGE | 50nmol | 1OD |
100nmol | 2OD | |
200nmol | 3OD | |
HPLC | 50nmol | 1OD |
100nmol | 2OD | |
200nmol | 5OD |
Generay Price Of Different Purification Opti♋ons And Pr🧔imer Length
1. Introduction aꦐnd price list of🐠 OPC purification primers
Purification with reverse-phase silica gel column wh💯ich can effectively remove salts. The purity can mওeet the needs of most molecular biology experiments.
Advantages
● Fast
● High throughput
● Cost-effective
Applications
PCR꧟ amplification, gene synthesis, DNA seq🎶uencing, NGS capture probes, NGS Multiplex PCR oligos.
Primer length | purification method | yield | Price($)/nt | Price($)/purified |
15-60nt | OPC | 2OD | 0.16 /nt | / |
15-60nt | OPC | 5OD | 0.20 /nt | / |
15-60nt | OPC | 10OD | 0.34 /nt | / |
15-60nt | OPC | >10OD | Inquiry | / |
2. Introduction and price list of PAGE purification primers
Using denaturing polyacrylamide gel electro🍒phoresis, oligo of different molecular weights migrate at different speeds during electrophoresis, allowing for separation. Finally, the target DNA is recovered from the gel.
Advantages
● Purity can be > 90%
● Effective for purification of long-stranded oligo
Applications
Mutation libraries, protein binding gel migration electrophoresis analys🅠is, modified oligos such as phosphate🥂 and biotin.
Primer Length | Purification Method | Yield | Price($)/nt | Price($)/purified Markup |
15-60nt | PAGE | 2OD | 0.21/nt | 5.00/ purified |
15-60nt | PAGE | 5OD | 0.35/nt | 7.00/ purified |
15-60nt | PAGE | 10OD | 0.45/nt | 9.00/ purified |
15-60nt | PAGE | >10OD | Inquiry | Inquiry |
* Markup prices are PAGE purification fees only |
3.Introduction and price list of HPLC purification primers
The purification of oligo with HPLC can achieve h🗹igh purity and is mainly used for short chain and modified oligo.
Advantages
● Purity can reach > 90%
● Remove N-1 short fragment effectively
● Purification for modifie🐽d oligo with hydrophobic groups
Applications
Point mutation, subcloning, diagnostic PCR protein binding g🍌el migration electrophoresis analysis, modified primers with hydrophobic groups.
Primer Length | Purification Method | Yield | Price($)/nt | Price($)/purified Markup |
15-60nt | HPLC | 2OD | 0.21/nt | 9.00 /purified |
15-60nt | HPLC | 5OD | 0.35 /nt | 12.00/ purified |
15-60nt | HPLC | 10OD | 0.45/nt | 13.50/ purified |
15-60nt | HPLC | >10OD | Inquiry | Inquiry |
61-90nt | HPLC | 2OD | 0.40nt | 11.00/purified |
61-90nt | HPLC | 5OD | 0.60/nt | 13.00/purified |
61-90nt | HPLC | 10OD | 0.90/nt | 16.00/purified |
61-90nt | HPLC | >10OD | Inquiry | Inquiry |
90-130nt | HPLC | 1OD | 0.60/nt | 13.00/purified |
90-130nt | HPLC | 2OD | 0.60/nt | 13.00/purified |
90-130nt | HPLC | 5OD | 0.90/nt | 16.00/purified |
90-130nt | HPLC | >5OD | Inquiry | Inquiry |
* Markup prices are HPLC purification fees only |
Contact Information
● By Email: dna@syouba.com
● By Phone: 86-21-67840801/0802