14-3-3η is a novel growth-promoting and angiogenic factor in hepatocellular carcinoma
Jian Shen, Fei Jiang, Ye Yang, Guangming Huang, Fuxing Pu, Qinqiang Liu,Lijun Chen, Liang Ju, Ming Lu, Fei Zhou, Chi Zhang, Xiagang Luo,XiaojunY🌠ang, Chengyu Jiao, Xiangcheng Li, Zhong Li, Yuan Li, Jianping Zhang
Journal of Hepatology November 2016Volume 65, Issue 5, Pages 953–962 DOI: //dx.doi.org/10.1016/j.jhep.2016.05.017……For cell transfection, the pcDNA-3.1-14-3-3-Flag plasmid that overexpressedꦯ both 14-3-3 and Flag was created by inserting the coding sequences of 14-3-3 (YWHAH, 741 bp) into pcDNA3.1 plasmid, followed by adding a Flag tag at its♉ N-terminal (Generay Biotech Co. Ltd, Shanghai, China).……